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. 2016 Jan 29;2:15007. doi: 10.1038/bdjopen.2015.7

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Copyright © 2016 British Dental Association/Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Area visualised by TRI/3D-BON. Micro-CT parameters were as follows: X-ray source, 85 kV/140 μA; rotation, 360°; exposure time, 17 s; voxel size, 50×50×50 μm (R-mCT; Rigaku). CT images were compiled and 3D images were rendered using TRI/3D-BON (Ratoc System Engineering). The software was used to obtain a 3D reconstruction of the sets of scans. From the overall 3D data set, a region of interest with a diameter of 4.8 mm and height of 1.3 mm was selected for analysis, which included the entire thickness of the calvarial bone.