Table 3. Senescence markers and their correlations between short-term versus long-term experiments (2A).
Distribution of markers | ||||
Short-term experiment | Long-term experiment | Correlation coefficient | P-value | |
Non-stressed | ||||
ROS, FI | 1559 (1356; 1734) | 1500 (1366; 2205) | 0.419 | 0.010 |
SAβ-gal, FI | 2973 (2445; 3732) | 3452 (2905; 4660) | -0.009 | 0.959 |
Stressed | ||||
ROS, FI | 2095 (1753; 2324) | 1835 (1553; 2205) | 0.426 | 0.009 |
SAβ-gal, FI | 4171 (3530; 5231) | 4090 (3417; 5205) | -0.006 | 0.972 |
N=40. Correlations are Pearson’s partial correlation coefficient, adjusted for batch. All mean markers of short-term experiments I and II were correlated with the same markers in the long-term experiment. FI: fluorescence intensity. ROS: mean fluorescence intensity peak reactive oxygen species; SAβ-gal: median fluorescence intensity peak senescence-associated β galactosidase.