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. Author manuscript; available in PMC: 2018 Aug 26.
Published in final edited form as: Nat Struct Mol Biol. 2018 Feb 26;25(3):225–232. doi: 10.1038/s41594-018-0036-6

Figure 5. Accurate H3K27 methylation and pluripotency established from Suz12 KO cells with both methylation and PRC2 binding erased.

Figure 5

a, Western blot of extracts from indicated cell lines probing levels of PRC2 subunits and H3K27 methylation levels. Experiment has been repeated at least three times in individual knockout clones with the same result. Uncropped Western blot images are shown in Supplementary Data Set 1.

b, ChIP-seq signals within a representative genomic region from indicated cell lines generated with H3K27me3 and Suz12 antibodies. ChIP-seq signal is sequence-depth normalized (reads per million mapped reads).

c, Images of embryos dissected at E13.5. Embryos are derived from injection of morulae aggregated with mCherry labeled WT mESCs (129B6F1), Suz12 KO or SUZ12 rescue (KO + SUZ12) cells. Scale bar: 5 mm.