Figure 5.

Lack of the −16kb enhancer of Fgf23 blunts the LPS‐induced increase in Fgf23 levels. Twelve‐week‐old wild‐type (WT) and Fgf23 ^−16KO female mice were injected with 10 mg/kg of LPS or vehicle (PBS). Tissues were collected 6 hours after injection. (A–H) Lumbar vertebra 5 (L₅) (A), femur shaft (F. Shaft) (B), and non‐osseous tissue (C–H) Fgf23 mRNA levels were measured by RT‐PCR. Bars represent the mean ± SD of 4 to 6 mice/group. Statistical comparisons were performed using Two‐Way ANOVA with multiple comparison test using the Benjamini‐Hochberg procedure. *, p<0.05 effect of treatment within the same genotype; #, p<0.05 effect of genotype within the same treatment, and “a”, p<0.05 magnitude of the difference (vehicle‐injection) is less in Fgf23 ^−16KO mice compared to WT mice. UD indicates that Fgf23 levels were undetectable by RT‐PCR.