Figure 6.

Lack of the −16kb enhancer of Fgf23 blunts the Tnfα‐ and IL‐1β‐induced increase in Fgf23 levels. Eight‐week‐old wild‐type (WT) and Fgf23 ^−16KO female mice were injected with 2 μg of recombinant Tnfα or vehicle, while their male littermates were injected with 50 ng/g of IL‐1β or PBS. The tissues were collected 3 hours after TNFα and 6 hours after IL‐1β injection. The Fgf23 mRNA levels were measured by RT‐PCR and represented here as the mean ± SD (n = 5 to 9 mice/group). Statistical comparisons were performed using two‐way ANOVA with multiple comparison test using the Benjamini‐Hochberg procedure. *p < 0.05 effect of treatment within the same genotype; #p < 0.05 effect of genotype within the same treatment, and “a”, p < 0.05 magnitude of the difference (vehicle‐injection) is less in Fgf23 ^−16KO mice compared with WT mice. UD indicates that Fgf23 levels were undetectable by RT‐PCR.