Figure 4.

Icotinib hydrochloride inhibits EGFR signaling pathway in Hela S3 cells.

Notes: Total EGFR, p-EGFR, total AKT, p-AKT, total ERK and p-ERK were detected using Western blot assays in Hela S3 cells 2 hours after combination treatment of radiation (A) or cisplatin (B) with IH (top). Histograms indicate the relative protein expression levels in the cells by grayscale analysis (bottom). Error bars, SD. *P<0.05, **P<0.01, and ***P<0.001 by two-tailed Student’s t-test; n=3 independent cell cultures.

Abbreviations: Ctrl, control; IH, icotinib hydrochloride; IR, ionizing radiation; Cis, cisplatin.