Fig 1. The absence of NKLAM impairs S. pneumoniae killing.

(A) WT and NKLAM-KO mice were infected by nasal administration of 10 x 10⁶ CFU of S. pneumoniae. The lungs were harvested at 24 or 48h, homogenized in sterile PBS and an aliquot was plated on blood agar plates to determine bacterial load. Instillation of sterile PBS was used as a control. Data represent mean ± SD of 1 of 3 independent experiments. (n = 8–11 total mice for each condition); * p < 0.05. (B) Bone marrow-derived neutrophils (BM-PMN) or C) macrophages (BMDM) were incubated with serum-opsonized S. pneumoniae at an MOI of 0.01. The percentage S. pneumoniae killed was determined relative to control cultures lacking neutrophils or macrophages. n = 3; p < 0.05.