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(A) mRNA isolated from the lungs of infected WT and NKLAM-KO mice was used to determine the relative expression of iNOS. Lung homogenates were used to assess iNOS protein expression. Beta actin was used as a loading control. (B) Quantitative PCR was used to determine the relative expression of MCP-1, MIP1α, IFNγ and NKLAM in infected lungs. The mRNA levels (mean ± SD) are expressed relative to PBS-treated mice. * p < 0.05; n = 8–11 mice per group.
