Table 2.
Responses values for particle size (nm), zeta potential (mV) and entrapment efficiency (%) of DS-NPs according to the half fractional factorial 2 (5-1) design
| Std order | Factors | Responses | |||||||
|---|---|---|---|---|---|---|---|---|---|
| DS(mg/mL) | TPP(mg/mL) | rate(mL/min) |
pH
- |
SPION/DS
- |
PS (nm) | ZP(mV) | EE (%) | ||
| S1 | - - - - + | 2.00 | 0.50 | 35.00 | 3.00 | 0.75 | 76 | +37.90 | 13.23 |
| S2 | + - - - - | 4.00 | 0.50 | 35.00 | 3.00 | 0.50 | 64 | +35.50 | 12.92 |
| S3 | - + - - - | 2.00 | 1.00 | 35.00 | 3.00 | 0.50 | 96 | +15.00 | 27.13 |
| S4 | + + - - + | 4.00 | 1.00 | 35.00 | 3.00 | 0.75 | 77 | +33.30 | 20.15 |
| S5 | - - + - - | 2.00 | 0.50 | 60.00 | 3.00 | 0.50 | 72 | +20.30 | 10.55 |
| S6 | + - + - + | 4.00 | 0.50 | 60.00 | 3.00 | 0.75 | 72 | +32.40 | 15.70 |
| S7 | - + + - + | 2.00 | 1.00 | 60.00 | 3.00 | 0.75 | 100 | +19.40 | 17.89 |
| S8 | + + + - - | 4.00 | 1.00 | 60.00 | 3.00 | 0.50 | 76 | +24.40 | 11.35 |
| S9 | - - - + - | 2.00 | 0.50 | 35.00 | 5.00 | 0.50 | 99 | +17.50 | 26.12 |
| S10 | + - - + + | 4.00 | 0.50 | 35.00 | 5.00 | 0.75 | 80 | +33.90 | 14.49 |
| S11 | - + - + + | 2.00 | 1.00 | 35.00 | 5.00 | 0.75 | 183 | +18.20 | 11.37 |
| S12 | + + - + - | 4.00 | 1.00 | 35.00 | 5.00 | 0.50 | 139 | +24.10 | 23.28 |
| S13 | - - + + + | 2.00 | 0.50 | 60.00 | 5.00 | 0.75 | 117 | +20.80 | 17.04 |
| S14 | + - + + - | 4.00 | 0.50 | 60.00 | 5.00 | 0.50 | 83 | +39.60 | 9.38 |
| S15 | - + + + - | 2.00 | 1.00 | 60.00 | 5.00 | 0.50 | 135 | +10.20 | 22.80 |
| S16 | + + + + + | 4.00 | 1.00 | 60.00 | 5.00 | 0.75 | 80 | +31.60 | 17.59 |
| S17 | 0 0 0 0 0 | 3.00 | 0.75 | 47.50 | 4.00 | 0.63 | 90 | +32.80 | 11.19 |
| S18 | 0 0 0 0 0 | 3.00 | 0.75 | 47.50 | 4.00 | 0.63 | 89 | +29.67 | 17.98 |
| S19 | 0 0 0 0 0 | 3.00 | 0.75 | 47.50 | 4.00 | 0.63 | 96 | +25.78 | 16.97 |
*
A- Dextran-spermine concentration (DS); B- tripolyphosphate concentration (TPP); C- tripolyphosphate addition rate (Rate); D- pH of dextran-spermine solution (pH); E- Super paramagnetic iron oxide nanoparticle/dextran-spermine mass ratio (SPION/DS); Particle size (PS); Zeta potential (ZP); Entrapment efficiency (EE).