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. 2018 Feb 26;12(2):e0006301. doi: 10.1371/journal.pntd.0006301

Fig 4. Expression of HGPRT-I, HGPRT-II and HGXPRT in the PF and BF cells.

Fig 4

(A) The steady state abundance of HGPRT-I, HGPRT-II and HGXPRT was determined in PF and BF cells by Western blot analysis of whole cell lysates. Densitometric analysis was performed using the Image Lab 4.1 software and the number beneath the blots represents the abundance of immunodetected proteins expressed as a percentage of the PF sample. (B) Cellular fractionation of PF and BF cells was used to distinguish between the localization of cytosolic HGPRT-I and glycosomal HGPRT-II. Antibodies against hexokinase and enolase were used to mark the cytosol and glycosome, respectively. The protein marker sizes are indicated on the left.