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. 2018 Feb 7;52(4):1198–1208. doi: 10.3892/ijo.2018.4267

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Copyright: © Tian et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Knockdown of MMP1 alters malignant behavior in vitro. (A) Efficacy of MMP1 CRISPR was demonstrated by western blotting. (B) Cell viability of MMP1 knockdown and control cells was detected by PrestoBlue, relative growth was represented by fluorescence at a wavelength of 570 nm. MMP1 knockdown cells grew more slowly on day 5 and 6 compared with control cells. (C) Cell motility was analyzed by wound healing assay, bar chart displayed the 36 h relative wound closure of SiHa and HeLa MMP1 knockdown and control cells. (D) Cell invasion was analyzed Transwell assay, images at 36 h are presented and bar chart displays the cell count per field from random three fields. Data are presented as the mean ± standard error (n=3; ^*P≤0.05 vs. dox-). MMP1, matrix metalloproteinase 1; sgRNA, single guide RNA; dox, doxycycline hyclate.