Figure 5.

Bcl-2 mRNA is a direct target of miR-15a. The 3′UTR fragment containing wild-type or mutant seed region of the miR-15a was cloned into a dual-luciferase reporter vector and the luciferase activity was analysed. (A) Cloning of the wild-type and mutant 3′UTR of the Bcl-2 gene. (B) Construction of the luciferase reporter plasmids. (C) The wild-type reporter vector exhibited a significant reduction of luciferase activity when co-transfected with miR-15a, whereas the mutation vector abrogated this inhibitory effect. ^*P<0.05 vs. the UTR-WT + CT group. Bcl-2, B-cell lymphoma 2; UTR, untranslated region; WT, wild-type; MU, mutant; CT, control.