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. Author manuscript; available in PMC: 2018 Mar 8.
Published in final edited form as: Arch Biochem Biophys. 2016 Jan 9;601:133–140. doi: 10.1016/j.abb.2016.01.006

Figure 4.

Figure 4

cMyBP-C protein expression in myocardium from wild-type (WT), heterozygous (HT) and homozygous (HO) cats determined using western blot analysis. A. Representative western blots probed with the C-pro antibody that primarily recognizes the C0 domain of cMyBP-C. The positions of cMyBP-C and α-actinin (loading control) are indicated. B. Left panel: Box-and-whiskers plot (median, 25-75 percentile box and min-max whiskers) summarizing data from western blots probed using the C-pro antibody with signal normalized to α-actinin. Right panel: The same data shown at left but plotted to show the mean of each group and disease status. Filled symbols, HCM affected cats; Open symbols, unaffected cats. C. Representative western blots probed with the K16 antibody (Santa Cruz) that recognizes internal regions of cMyBP-C. D. Left panel: Summary data from western blots probed using the K16 antibody with signal normalized to α-actinin. Right panel: The same data shown at left but plotted to show the mean of each group and disease status. E. Representative western blots probed with an antibody specific for the A31P substitution. F. Left panel: Summary data from western blots probed using the A31P antibody with signal normalized to α-actinin. Right panel: The same data shown at left but plotted to show the mean of each group and disease status.