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. Author manuscript; available in PMC: 2018 Mar 8.
Published in final edited form as: Arch Biochem Biophys. 2016 Jan 9;601:133–140. doi: 10.1016/j.abb.2016.01.006

Figure 5.

Figure 5

Immunofluorescence localization of cMyBP-C in cat myocardium. Top: Immunofluorescence staining using the C-pro antibody showed the expected doublet pattern of cMyBP-C localization (green) in myocardium from wild-type (A), A31P heterozygous (B), and A31P homozygous (C) cats. Staining for α-actinin (red) was used to label sarcomere Z-lines as a reference. Bottom: Immunofluorescence localization using the A31P antibody showed that mutant A31P cMyBP-C is also properly incorporated into sarcomeres as demonstrated by the characteristic doublet pattern of cMyBP-C localization (green) in myocardium from heterozygous (E) and homozygous (F) cats but not wild-type (D) cats that lack A31P cMyBP-C. α-actinin localization (red) was used to label sarcomere Z-lines as a reference.