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. Author manuscript; available in PMC: 2019 Apr 1.
Published in final edited form as: J Pineal Res. 2018 Jan 9;64(3):10.1111/jpi.12463. doi: 10.1111/jpi.12463

Figure 3. Melatonin prevents HOCl mediated corrin destruction and CNCl generation from cyanocobalamin.

Figure 3

(A) HPLC analysis of cyanocobalamin-HOCl reaction mixtures in presence/absence of 1:1 melatonin:HOCl molar ratio. Chromatograms were obtained at 360 nm. Relative amount of cyanocobalamin remaining was calculated from the area under the curve for the cyanocobalamin peak, and is shown in inset. The data shown is a representative of three independent experiments and the error bars are represent the standard error of measurements. (B) Pseudo-first order rate constants of HOCl-mediated cyanocobalamin destruction, plotted as a function of melatonin:HOCl molar ratios. The data points are the average of three independent experiments, and the error bars represent the standard error of measurements. (C) cyanocobalamin (100 µM) was treated with 3000 µM of HOCl in presence/absence of melatonin (3000 µM) and CNCl generation was assayed colorimetrically as detailed under Materials and Methods section. The data are representative of three independent experiments with the error bars representing the standard error measurements.