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. Author manuscript; available in PMC: 2019 Apr 1.
Published in final edited form as: J Immunol Methods. 2018 Jan 31;455:41–54. doi: 10.1016/j.jim.2018.01.009

Fig. 4.

Fig. 4

Fig. 4

Fig. 4

Examples of IgG2 (subjects 10, 11 and 12) and IgG4 (subjects 13, 14 and 15) levels measured in serial dilutions of plasma samples from 6 Mozambican adults against a panel of 10 Plasmodium falciparum antigens using a triple (mouse anti-human IgG2 at 1:500 plus goat anti-mouse IgG-Biotin at 1:1,000) and double sandwich (anti-human IgG4-Biotin at 1:8,000), respectively, and incubated with streptavidin-PE at 1:1,000. Data obtained using a triple sandwich (mouse anti-human at 1:8,000 plus goat anti-mouse IgG-Biotin at 1:1,000) to measure IgG4 in a pool of plasmas from hyperimmune adults are also shown.