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. Author manuscript; available in PMC: 2019 Apr 1.
Published in final edited form as: J Immunol Methods. 2018 Jan 31;455:41–54. doi: 10.1016/j.jim.2018.01.009

Table 3.

Details of secondary-tertiary antibodies and Streptavidin-Phycoerythrin (SA-PE) reagents.

Ig Secondary antibody Tertiary antibody SA-PE
IgG1 anti-IgG1-Biotin (Abcam, ab99775) 1:4000 - Streptavidin-R-phycoerythrin (Sigma, 42250) 1:1000
IgG2 mouse anti-human IgG2 (Thermo Fisher, MA1-34755) 1:500 goat anti-mouse IgG-Biotin (Sigma, B7401-1ML) 1:1000
IgG3 anti-IgG3-Biotin (Sigma, B3523) 1:1000 -
IgG4 anti- IgG4-Biotin (Sigma, B3648) 1:8000 -
IgM anti-IgM-Biotin (Sigma, B1265) 1:1000 -
Antigen-specific IgE Mouse anti-human IgE (Abcam, ab99834) 1:250 goat anti-mouse IgG-Biotin (Sigma, B7401-1ML) 1:125
Total IgE anti-IgE-Biotin (Thermo Fisher, A18803) 1:2000 -