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. 2018 Mar 8;9:1009. doi: 10.1038/s41467-018-03394-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — Genetic disruption of Malat1 or Hdac9 improves experimental aortic aneurysm. a Explanted VSMCs from Fbn1^C1039G/+ mouse aortas demonstrate increased colocalization of Hdac9–Brg1 as well as Malat1–Brg1 when compared to wild-type cells. Global Pearson’s colocalization (GPC) of spatial overlap between Hdac9–Brg1 and Malat1–Brg1 is shown. Bar = 2.5 µM b Identification of Hdac9–Malat1–Brg1 complex in wild type and Fbn1^C1039G/+ by confocal microscopy of ascending aortic samples. Bar = 40 µM c QPCR of Hdac9, Malat1, and Brg1 demonstrate upregulation in Fbn1^C1039G/+ aortas, bar graphs are presented as mean with error bars (±S.D.) n = 6 animals, 6 months of age (d) Improved quantitative aortic dimensions in Fbn1^C1039G/+:Malat1^−/− and Fbn1^C1039G/+:Hdac9^fl/fl:Tagln-cre mice when compared to Fbn1^C1039G/+ mice. Ultrasound of aortic root (Red) and ascending aortic (Black) quantification of wild-type (n = 18), Malat1^−/− (n = 18), Hdac9^fl/fl:Tagln-cre (n = 17), Fbn1^C1039G/+ (n = 24), Fbn1^C1039G/+:Malat1^−/− (n = 19), and Fbn1^C1039G/+:Hdac9^fl/fl:Tagln-cre (n = 15; One-way ANOVA, *p < 0.05, **p < 0.01, ***p < 0.001). Aortic dimensions at 6 months and aortic growth between 16 and 24 weeks are shown. Representative images of parasternal long axis systolic ultrasound images of the aortic root of 6-month old mice. Red arrows denote aortic root, white arrows denote ascending aorta. Bar = 1 mm (e) Malat1 and Hdac9 deficiency rescues anatomic and cytoskeletal defects in Fbn1^C1039G/+ aortas. Representative photomicrographs of six month old latex-injected wild-type, Malat1^−/−, Hdac9^fl/fl:Tagln-cre, Fbn1^C1039G/+, Fbn1^C1039G/+:Malat1^−/−, and Fbn1^C1039G/+:Hdac9^fl/fl:Tagln-cre hearts and ascending aortas (left column, Bar = 1 mm, middle and right column, Bar = 40 µM). Red arrows indicate the ascending portion of the aorta. Aortas stained with Verhoeff-Van Gieson stain (center panels), and immunofluorescence of F-actin (right panels). The aortic adventitia faces left while the aortic lumen is oriented to the right. Quantification of aortic architecture and F-actin staining, (One-way ANOVA, *p < 0.05, **p < 0.01, ***p < 0.001). Full-length western blots presented in Supplementary Fig. 8