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. 2018 Mar 8;8:4171. doi: 10.1038/s41598-018-22480-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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All tumor types show comparable mitochondrial membrane potential (MMP), and low vascular oxygenation (SO₂) enhances differences between tumor and normal MMP. (a) TMRE uptake at 60 minutes (TMRE₆₀) and SO₂ in non-tumor and tumor window chambers. Tumor regions are shown in the transmission images as areas of increased absorption contrast. (b) Mean distribution of all TMRE₆₀ pixels for each tissue type. (c) Mean distribution of TMRE₆₀ for each tissue type for distinct levels of regional SO₂. Group numbers shown in legend. N.T. = Non-tumor. AUC = area under curve. Error bars = SE. *is p < 0.05.