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. 2018 Mar 8;8:4171. doi: 10.1038/s41598-018-22480-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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4T1 tumor and peri-tumoral area maintain distinct metabolic and vascular phenotypes. Metabolic and vascular endpoints were quantified for the tumor and peritumoral area (PA) of all tumor types (67NR, 4T07, 4T1). (a) Transmission images were segmented into tumor and peritumoral area (PA) regions. (b) Comparison of mean vascular features in non-tumor tissue and tumor and PA regions of all tumor types. SO₂ = vascular oxygenation. (c) TMRE₆₀ in the tumor and PA of all tumor types. (d) 2-NBDG₆₀/R_D in the tumor and PA of all tumor types. N.T. = Non-tumor. AUC = area under curve. Error bars = SE. n = 12-14 (b) or as shown in legend (c,d). *is p < 0.05 vs. N.T. (b). ◊ is p < 0.05 tumor vs. PA for same tumor line (b). *is p < 0.05 tumor vs. PA for same tumor line (c,d).