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. 2018 Mar 8;8:4176. doi: 10.1038/s41598-018-22673-3

Figure 1.

Figure 1

Basic activity of PARP3 in DNA ADP-ribosylation. (A) Schematic representation of the DNA duplexes used. The asterisk marks the [32P]-label. (B) Activity of 0.1 µM PARP3 on gap1 in the absence (lanes 5–7) or presence of 10 mM EDTA (lanes 2–4), 1, 2, 5 and 10 mM MgCl2 (lanes 8–23) or 1, 2, 5 and 10 mM spermine (lanes 24–39). The reactions were performed using increasing concentration of NAD+ (1, 10, 100 and 1000 µM). (C) The reaction yield of modified DNA using various initial DNA substrates catalysed by 0.1 µM PARP3 with 100 µM NAD+.