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. 2016 Nov 21;15(3):246–259. doi: 10.1038/cmi.2016.49

Figure 6.

Figure 6

p38α deletion affected cytokine production by DCs in response to TLR stimulation. (a–c) Different subsets of BM-derived DCs from the WT and p38αΔDC mice were used to measure cytokine productions in response to the indicated TLR ligand stimulation. The production of IL-12p40 or IL-12p70 by CD24+ cDCs (a, b) and IL-12p40 by CD24− cDCs (c) after stimulation by TLR agonists for 36 h was measured by ELISA. Non-stimulated DCs were used as the negative controls. (d–f) Production of IL-12p40 /p70 by CpG-triggered splenic CD8+ cDCs and IL-12p40 by CD8− cDCs was examined by ELISA at 36 h. The medium used was RPMI 1640 supplemented with 10% FBS, 1% P/S (penicillin and streptomycin) and GM-CSF (20 ng/ml). (a–f) The results were representative of three independent experiments with at least two repeats. The data are presented as the mean±s.e.m. *P<0.05; **P<0.01; ***P<0.001.