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. 2017 Feb 13;15(3):272–281. doi: 10.1038/cmi.2016.67

Figure 7.

Figure 7

HBV infection inhibited TRIM25 expression. (a) Total RNA was extracted from PBMCs isolated from 28 healthy controls and 29 HBV patients. The TRIM25 expression level was analyzed using Q-PCR. (b) HepG2 cells were treated with serum from healthy controls or HBV patients for 24 h, and Q-PCR was performed to analyze the TRIM25 mRNA level. (c) Total RNA was extracted from HepG2 and HepG2.2.15 cells, and the TRIM25 expression level was analyzed using Q-PCR. (d) HepG2 cells were either treated with IFNa or untreated and either cultured with HepG2.2.15 supernatant or normal culture medium. After 8 h, the TRIM25 mRNA level was analyzed using Q-PCR. Data from three independent experiments were pooled and are shown as the means±s.d. *P<0.05, **P<0.01.