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. 2018 Mar 8;8:4189. doi: 10.1038/s41598-018-22635-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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HSP27 inhibitor decreased the expression of inflammation-related molecules. (A) cDNA was synthesized from the total RNAs extracted from mouse lungs exposed to X-rays and subjected to quantitative real-time PCR analysis for inflammation-related molecules. (B) Immunohistostaining of TGF-β (B) and IL-1b (C) using mice lung tissues. Quantification of stained tissue was presented as mean ± standard error. *P < 0.05, **P < 0.01 and ***P < 0.001 versus control; ^#P < 0.05, ^##P < 0.01 and ^###P < 0.001 vs. IR. Control, untreated; IR, 75 Gy irradiation (IR); IR + J2, irradiation + 15 mg/kg J2; IR + AMI, irradiation + 100 mg/kg amifostine; J2, 15 mg/kg J2 only and AMI, 100 mg/kg amifostine only.