Figure 6.

Effect of HSP27 inhibitor on ROS generation and inflammasome activation. (A) L132 cells were pretreated with J2 (2 uM) or NAC (10 mM) for 3 h and then exposed to X-rays at 10 Gy for 6 h. Cells were stained with DCF-DA and observed under fluorescent microscope. (B) Mice were sacrificed at 2 weeks after irradiation, mice plasma were centrifuge at 10,000 rpm for 5 min to remove insoluble particles. The content of H₂O₂ within the plasma was measured at absorbance at 560 nm. Quantitative real-time PCR for inflammasome-related genes. cDNA was synthesized from the total RNAs extracted from irradiated L132 cells (C) and mouse lungs (D) exposed to X-rays and subjected to RT-PCR analysis. (E) L132 cells were pretreated with or without J2 (2 uM) and then exposed to x-rays at 10 Gy for 24 h. Caspase-1 activity from cell lysates was measured at absorbance at 405 nm.