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. 2018 Mar 8;8:4189. doi: 10.1038/s41598-018-22635-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Gross morphological, histopathological and micro-CT analysis in HSP27 transgenic mice. (A) Representative gross finding. Mice were sacrificed at two weeks after irradiation. Four-percent paraformaldehyde was instilled via trachea, and lungs were immersed in fixation solution and photographed after complete fixation. (B) Haematoxylin and eosin-stained lung sections. (C) Graphs show quantification of inflammation score. (D) Representative micro-CT images of lungs of irradiated and control mice. Horizontal (top row), trans-axial (middle row), and 3D micro-CT (bottom row) images acquired at two weeks after irradiation. (E) Quantification of volume of normal left lung was presented as mean ± standard error. **P < 0.01 versus BL6 IR.