Fig. 4.

SYK-DC–CTLs exhibit specific cytotoxicity against carboplatin-resistant RB-Y79 cells in vitro. The cell viability of RB-79-S and RB-Y79-R cells in media containing carboplatin was measured by trypan blue staining (a) and PI staining (b). c Cytotoxicity and apoptosis analysis of GFP-DC–CTL cells, Ag-DC–CTL cells and SYK-DC–CTL cells against RB-Y79-R cells were detected by flow cytometry. The figures showed the proportion of dead RB-Y79-R cells (top panel) and apoptotic RB-Y79-R cells (bottom panel). d Cytotoxicity of GFP-DC–CTL cells, Ag-DC–CTL cells and SYK-DC–CTL cells against RB-Y79-R cells. e Quantification of apoptotic RB-Y79-R cells after co-culture with GFP-DC–CTL cells, Ag-DC–CTL cells or SYK-DC–CTL cells. Data were given as mean ± SEM from three independent experiments. *p < 0.05, **p < 0.01