Fig. 4.

The effect of lichen-derived chemicals on the cytosolic level of β-catenin (a) and phospho-β-catenin (b) and Axin2 (c) and the nuclear level of β-catenin (d) in HCT116 cells after 48 h incubation. Representative immunoblots are presented above graphs. Data were normalized against the level of β-actin (cytosolic proteins) or lamin (nuclear proteins). Fold of change in relation to control cells treated with the vehicle where protein level was arbitrarily expressed as 1 was calculated and mean values ± SEM from two independent experiments are shown. Asterisk above bars denotes statistically significant changes, p ≤ 0.05