Figure 1.

Interrogation of neuronal circuitry using chemogenetics: local intracranial administration of the activating ligand. Intracranial injection of DREADD ligands can be used to selectively manipulate neuronal circuits and behavioural output. In this sagittal rat brain schematic, an AAV‐encoding non‐conditional DREADD is injected into the NAcs, and intracranial guide cannulas are implanted above the VTA. Intracranial infusion of the DREADD ligand into VTA will change the activity of DREADD‐expressing terminals in VTA.