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. 2018 Mar 9;38(2):BSR20171255. doi: 10.1042/BSR20171255

Figure 4. mD1R improved crypt maturation after irradiation.

Figure 4

(A) Normal mice were irradiated by TBI with 12 Gy of γ-ray and injected i.p. with PBS or 100 μg of mD1R every day from the first day after the irradiation. Three days post the irradiation, the intestine of the mice was collected and subjected to H&E staining. The Paneth cells were indicated with arrowheads, and the number of Paneth cells was counted and compared between the two groups. (B) Total RNA was prepared from the intestine tissue in (A). The expression of Defa1 mRNA in intestine was determined by using real time RT-PCR. (C) The intestine was collected from mice in (A) on day 3 and 22 after the irradiation. The tissue sections were stained with Alcian Blue/Fast red. The number of Goblet cells per villus was compared; bars = mean ± SD (n=5); **P<0.01, ***P<0.001.