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. 2018 Mar 9;38(2):BSR20171430. doi: 10.1042/BSR20171430

Figure 4. miR-139-5p targets c-Jun expression in cardiomyocytes.

Figure 4

(A–D) NRCMs were treated as indicated. Levels of c-Jun, IGF-1R, MyoCD, Wnt1, and β-catenin (A) and the phosphorylation status of Akt (B) were detected using Western blotting. The relative expression intensity (B,D) was obtained from three independent experiments were performed. (E–I) siRNAs for knocking down (KD) c-Jun expression were co-transfected into to NRCMs with the miR-139-5p inhibitor or the inhibitor control RNA for 48 h. Expression of c-Jun was determined using Western blotting (E) and the relative expression of c-Jun was quantitated (F). F-actin and nuclei were stained with Texas Red-Phalloidin and DAPI, respectively. Scale bars: 100 μm (G). Cell surface area was quantitated from at least 30 cells in three independent experiments (H). Expression of ANP was quantitated by using real-time PCR and normalized to the level of 18S rRNA (n=3 per group) (I). Data are indicated as the mean ± S.E.M., *P<0.05 and **P<0.01 compared between the indicated groups.