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. 2017 Sep 22;32(3):588–596. doi: 10.1038/leu.2017.261

Figure 2.

Figure 2

RNA sequencing reveals an IL4 signature in MLL-AF9 leukemia cells enriched for apoptosis-related gene expression. c-Kit+ MLL-AF9 AML cells were cultured with mIL4 and mIL3 or mIL3 only (control). (a) RNA sequencing of murine AML cells stimulated for 18 h with mIL4. A two-group comparison between control and IL4-treated AML cells revealed an IL4-induced signature of 390 differentially expressed genes (FDR<0.05, P<0.001). Genes in the heatmap are ordered by hierarchical clustering. (b) Gene set enrichment analysis (GSEA) showing that the IL4 gene expression signature in AML cells is enriched for Caspase and death pathways (Biocarta). (c) Apoptosis and (d) cell cycle analysis of leukemia cells treated with mIL4 for 3 days (n=3). (e) Output cell number of 10 000 seeded c-Kit+ Tp53−/− MLL-AF9 leukemia cells treated with mIL4 or control for 3 days (n=3). (f) Apoptosis analysis of c-Kit+ Tp53−/− MLL-AF9 leukemia cells treated with mIL4 or control for 3 days (n=3). ES, enrichment score; FDR, false discovery rate; NES, normalized enrichment score. ****P<0.0001.