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. 2017 Sep 22;32(3):588–596. doi: 10.1038/leu.2017.261

Figure 7.

Figure 7

IL4 inhibits human AML cells by inducing apoptosis. Human leukemia cells were cultured for 3 days with hIL4 or without hIL4 (control). (a–b) MA9 leukemia cells treated with hIL4. (a) Annexin V and (b) Caspase-3 staining of MA9 cells (n=3). (c–e) Primary AML mononuclear cells (MNCs) from patients and NBM CD34+ cells were treated with hIL4 (n=3). (c) Output cell number of seeded AML MNCs following IL4 treatment. Output cell number is normalized to the control within samples. (d) Apoptosis analysis of MNCs from two AML samples (AML 2 and 8). (e) Output cell number of seeded NBM CD34+ cells following IL4 treatment. Output cell number is normalized to the control within samples. (f) FACS plots showing CD34 and CD38 staining of representative human CD34+ AML and NBM samples. Sorting of CD34+CD38− cells was performed according to the depicted gates. (g) Output cell number of seeded CD34+CD38− AML and NBM cells following treatment with hIL4. Output cell number is normalized to the control within samples. *P<0.05, **P<0.01, ***P<0.001. †Patient with an MLL-AF9 fusion gene.