Table 1. Affinities and Dynamic Range of LUMABS Sensors.

target antibody	sensor name	Ab binding elementa	monovalent Kd.Abb	sensor Kd.app	sensor DR
trastuzumab	TRAS-LUMABS-1	QLGPYELWELSH	0.29 ± 0.01 μM	303 ± 15 nM	59 ± 4%
	TRAS-LUMABS-2	LWGPYEWWELHH	1.6 ± 0.3 μM	0.6 ± 0.2 μM	20 ± 1%
	TRAS-LUMABS-3	LWGPYEWWEFHH	1.2 ± 0.2 μM	0.16 ± 0.05 μM	31 ± 2%
obinutuzumab	CD20-LUMABS-1	YNCEPANPSEKNSPSTQYCYSI	7 μMd	0.54 ± 0.13 nM	23 ± 1%
	CD20-LUMABS-2	YNCAPATPSEKNSPSTQYCYSI	N.D.	0.23 ± 0.06 nM	26 ± 1%
rituximab	CD20-LUMABS-1	YNCEPANPSEKNSPSTQYCYSI	28 μMd	3.9 ± 0.7 μM	22 ± 1%
	CD20-LUMABS-2	YNCAPATPSEKNSPSTQYCYSI	N.D.	N.B.	N.B.
cetuximab	CTX-LUMABS-1	CQFDLSTRRLKC	0.27 ± 0.01 μMc	376 ± 18 nM	47.3 ± 0.6%
	CTX-LUMABS-2	CVFDLGTRRLRC	61 ± 2 nMc	55 ± 3 nM	60.0 ± 0.8%
	CTX-LUMABS-3	AVFDLGTRRLRA	N.D.	N.B.	N.B.

^aEpitope, mimotope, or meditope as described in the text. For exact sequences of the proteins used in this study, refer to the Supporting Information.

^bPer binding site.

^cSee ref⁴³

^dSee ref (30). DR dynamic range. N.D. not determined. N.B. no binding.