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. 2018 Mar 4;7(1):1442985. doi: 10.1080/20013078.2018.1442985

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© 2018 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group on behalf of The International Society for Extracellular Vesicles.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — A protocol for the purification of EVs using a lectin-bead approach.

Following a lectin microarray screen, STL was found to bind to uEVs with greater specificity than urine glycoproteins [48]. Incubation of undiluted urine with biotinylated STL and streptavidin-conjugated Dynabeads allowed for magnetic recovery of uEVs.