Table II.
Target gene | Primer sequences |
---|---|
Perforin | |
Forward | 5′-GAGGAGAAGAAGAAGAAGCACAA-3′ |
Reverse | 5′-AGGGGTTCCAGGGTGTAGTC-3′ |
Granzyme B | |
Forward | 5′-CCAGCAGTTTATCCCTGTGAA-3′ |
Reverse | 5′-CACCTCTTGTAGTGTGTGTGAGTG-3′ |
NKP44 | |
Forward | 5′-TCCAAGGCTCAGGTACTTCAAAG-3′ |
Reverse | 5′-GGGCGGGTACTGGCATCT-3′ |
NKG2D | |
Forward | 5′-TCCATTCTCTCACCCAACCTAC-3′ |
Reverse | 5′-CTCTGTTCCTGGCTTTTATTGA-3′ |
β-actin | |
Forward | 5′- TTGCCGACAGGATGCAGAA-3′ |
Reverse | 5′- GCCGATCCACACGGAGTACT-3′ |
NKP44, natural-killer protein 44; NKG2D, natural-killer group 2, member D.