Figure 1.

Generation of PLAC1-specific TCR-engineered CD8+ T cells. (A) Schematic illustration of the lentiviral vector encoding an anti-PLAC1 TCR expression cassette. TCRα- and TCRβ-chains were linked with a 2A peptide sequence. (B) CD8+ T cells, selected from peripheral blood mononuclear cells with a negative selection procedure using magnetic beads, were analyzed using flow cytometry for CD3 and CD8 expression via FITC-labeled mAb against human CD8 and PerCP-Cy5.5-labeled mAb against human CD3. (C) CD8+ T cells transduced with PLAC1-specific TCR or negative control or untransduced were stained with PE-labeled PLAC1-multimer (PE-multimer) and FITC-labeled CD8 mAb (CD8-FITC). The results are representative of three independent experiments. PLAC1, placenta-specific 1; TCR, T cell receptor; CD8+ T cell, cytotoxic T cell; CD, cluster of differentiation; FITC, fluorescein isothiocyanate; PE, phycoerythrin; mAb, monoclonal antibody.