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. 2018 Feb 16;15(4):5924–5932. doi: 10.3892/ol.2018.8075

Figure 2.

Figure 2.

Identification of PLAC1 and HLA-A2 serotype-positive breast cancer cell lines. (A) Immunofluorescence staining with rabbit anti-PLAC1 primary antibody or a rabbit IgG and PE-conjugated secondary antibody in the human non-metastatic breast cancer cell line MCF-7 and triple-negative breast cancer cell line MDAMB-231. The scale bar indicates 50 µm. (B) MCF-7 and MDAMB-231 cells were analyzed using flow cytometry for HLA-A2 expression using the PE-labeled HLA-A2 antibody and PE-labeled isotype control. The results are representative of three independent experiments. PLAC1, placenta-specific 1; HLA, human leukocyte antigen; PE, phycoerythrin; IgG, immunoglobulin G.