Figure 5.

Mechanism of enhanced activation of TCR-transduced CD8+ T cells. Cell lysates of TCR- or NC-transduced CD8+ T cells were analyzed by western blot analysis for the expression of PI3Kγ, AKT, p-AKT, ERK1/2, p-ERK1/2, JNK, p-JNK, p38, p-p38, NF-κB and GAPDH. The results are representative of three independent experiments. NC, negative control; TCR, T cell receptor; CD8+ T cells, cytotoxic T cells; PI3Kγ, phosphoinositide 3-kinase γ; AKT, protein kinase B; ERK1/2, extracellular signal-regulated kinase; JNK, c-Jun N-terminal kinases; NF-κB, nuclear factor κB; p-, phosphorylated.