Figure 3.

MAP30 induces apoptosis and arrests cell cycle at S-phase in glioblastoma cells. (A) U87 and (B) U251 cells were treated with MAP30 (1.4 and 2.7 µM) or PBS for 24 h, and alterations in cell morphology were determined following Hoechst 33342/PI staining and fluorescence microscopy (×400 magnification). (C) U87 and (D) U251 cells, following treatment with MAP30 (1.4 and 2.7 µM) or PBS for 24 h, underwent annexin V-PI double staining and were analyzed by flow cytometry. (E) U87 cells following treatment with MAP30 (1.4 and 2.7 µM) or PBS were stained with PI and analyzed using flow cytometry. MAP30 was identified to arrest the cell cycle at S-phase. (F) The annexin V-PI double staining results indicated that MAP30 promotes apoptosis of U87 and U251 cells in a dose-dependent manner. (G) The PI staining results indicated that MAP30 arrests the cell cycle at S-phase in a dose-dependent manner. All experiments were repeated three times, and images are representative. MAP30, Momordica anti-human immunodeficiency virus protein of 30 kDa; PI, propidium iodide.