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. 2018 Jan 16;15(3):3944–3950. doi: 10.3892/ol.2018.7812

Figure 2.

Figure 2.

KQ-induced apoptosis in HeLa cells. (A) Morphological changes of HeLa cells under phase contrast microscopy. HeLa cells were treated with KQ (6.25, 12.5 and 25 µg/ml) for 24 h at 37°C. Morphological changes of HeLa cells were observed using phase-contrast microscopy (magnification, ×20) and imaged were captured. (B) Effect of KQ on HeLa cells determined using Hoechst 33258 staining. HeLa cells were treated with KQ (6.25, 12.5 and 25 µg/ml) for 24 h at 37°C. Subsequently, cells were stained with Hoechst 33258 dye and the images were captured via a laser-scanning confocal microscope (magnification, ×20). (C) Flow cytometric analysis of apoptotic rate of HeLa cells. Cells were incubated with KQ at different concentrations (6.25, 12.5 and 25 µg/ml) for 24 h at 37°C. Cells were stained with Annexin V-fluorescein isothiocyanate/propidium iodide. (D) Total apoptotic rate of HeLa cells. K, kaempferol 3-O-β-(2″-acetyl) galactopyranoside; Q, quercetin; Apop., apoptosis; Con, control.