Figure 3.

(A) Effect of KQ on HeLa cells determined using DCFH₂-DA staining. HeLa cells were treated with KQ (6.25, 12.5 and 25 µg/ml) for 24 h at 37°C and then stained using DCFH₂-DA. Cells were analyzed using a flow cytometer. (B) Effect of KQ on HeLa cells of rhodamine 123 staining. HeLa cells were treated with KQ (6.25, 12.5 and 25 µg/ml) for 24 h at 37°C and then stained using rhodamine 123. The mitochondrial depolarization pattern of cells was observed using a laser-scanning confocal microscope (magnification, ×200) and images were captured. K, kaempferol 3-O-β-(2″-acetyl) galactopyranoside; Q, quercetin; DCFH₂-DA, 2′,7′-dichlorodihydrofluorescein diacetate; Con, control.