Table I.
Primer and probe sequences used for reverse transcription-quantitative polymerase chain reaction.
| Gene | Primer (5′-3′) | Roche universal probe no. |
|---|---|---|
| TXNIP | F: CTTCTGGAAGACCAGCCAAC | 85 |
| R: GAAGCTCAAAGCCGAACTTG | ||
| PGA3 | F: CCCGTCTTTGACAACATCTG | 81 |
| R: ATCACCACGCTGCCACTC | ||
| TNNT1 | F: GACTACATGGGGGAGGAACA | 17 |
| R: GCCATCAGGTCGAACTTCTC | ||
| SCRG1 | F: TGTTACTGCAACTTCAGCGAAT | 44 |
| R: TTGCAAGGAATCACGAAAGA | ||
| INHBE | F: CAGGGAGTGTGGCTCCAG | 52 |
| R: TGTAGGCTGAAGTGGAGTCTGT | ||
| GLI1 | F: CAGGGAGGAAAGCAGACTGA | 76 |
| R: ACTGCTGCAGGATGACTGG |
TXNIP, thioredoxin interacting protein; PGA3, pepsinogen 3; TNNT1, troponin T type 1 (skeletal, slow); SCRG1, stimulator of chondrogenesis 1; INHBE, inhibin-βE; GLI1, GLI family zinc finger 1; F, forward; R, reverse.