Figure 5.

Mutator effects of cancer-associated Polε variants in diploid yeast strains. Mutation rates were measured in diploid yeast strains heterozygous or homozygous for pol2 mutations mimicking the human POLE variants. Relative mutation rates are shown compared with wild type (WT). The human variants are listed on the x-axis with the analogous yeast substitutions in parentheses. Exo⁻, exonuclease-deficient variant encoded by the pol2-4 allele. (A) Can^R mutation was measured in strains containing a single copy of the CAN1 gene as described in Materials and Methods. (B) His⁺ reversion was measured in strains homozygous for the his7-2 allele. Asterisks indicate P < 0.05 by Wilcoxon–Mann–Whitney test compared with WT. Data are from Table S6 in File S1. ^aData from Kane and Shcherbakova (2014).