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Clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9-mediated gene editing in zebrafish. (A) Schematic illustrating the no nuclear localization signal (NLS) Cas9 protein, various NLS-fused Cas9 proteins, and Cas9 mRNA. (B) tyr target 1 and gol target 1 were as previously described, tyr target 2 was located in the second exon of tyr, and gol target 2 in the sixth exon of gol.
