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Multiplex knockout efficiency of various nuclear localization signal (NLS)-fused Cas9 proteins and N-Cas9-C mRNA. Survival rates of zebrafish embryos at 24 and 48 hr postfertilization (hpf) (A) and T7 Endonuclease I (T7E1) assays of the rates of mutagenesis for each site (B). The phenotypes observed were wild-type (WT) (C), severe mosaic hypopigmentation (D), and null-like (E). (F) Proportions of embryos with each phenotype. Scale bars: 0.2 mm.
