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. 2018 Jan 10;8(3):845–857. doi: 10.1534/g3.117.300452

Figure 5.

Figure 5

srpHemo-GAL80 blocks the effect of GAL4 drivers on plasmatocytes. (A–F) Confocal images of fixed (A and B) and live (C–F) samples. (A–A”) Stage 11 srpHemo-H2A::3xmCherry/+, tub-GAL4 UAS-CD8::GFP/+, embryo showing the ubiquitous GAL4-dependent labeling of cell membranes by (A and A’) UAS-CD8::GFP, including in plasmatocytes labeled by the nuclear mCherry (A–A”). (B–B”) Stage 11 srpHemo-H2A::3xmCherry srpHemo-GAL80/+, tub-GAL4 UAS-CD8::GFP/+, embryo demonstrates that the expression of GAL80 in plasmatocytes labeled by nuclear mCherry (B”) leads to the suppression of CD8::GFP (B,B’). (C–C”) First-instar srpHemo-H2A::3xmCherry/+, srp-GAL4 UAS-2xeGFP/+, larva shows that plasmatocytes labeled by the nuclear mCherry also express cytoplasmic GFP. However, in a larva also carrying srpHemo-GAL80, the plasmatocytes (D) no longer express the GAL4-controlled GFP (D’ and D”). (E–E”) Legs of srpHemo-H2A::3xmCherry/+, srp-GAL4 UAS-2xeGFP/+, adults; GFP is expressed in plasmatocytes under GAL4 control (E’ and E”), but not in the presence of srpHemo-GAL80 (F’ and F”). Scale bars correspond to 20 μm in (A and B), 20 μm in (C and D), and 50 μm in (E and F).