Skip to main content
. 2017 Dec 20;208(3):1037–1055. doi: 10.1534/genetics.117.300554

Figure 6.

Figure 6

The transcriptional repressor, Hap1, is required for Set4 binding to the promoters of ergosterol genes under hypoxic conditions. (A and B) Relative transcript levels of ERG11 and ERG3 were determined following 8 hr of hypoxia in WT, hap1Δ, and tup1Δ strains using qRT-PCR analysis. Transcript levels were set relative to the WT strain and expression levels were normalized to RDN18-1. *** P < 0.005, **** P < 0.0001. (C and D) Schematics of ERG11 and ERG3 loci with the specified positions of ChIP probes. (E and F) ChIP analysis of Hap1 under hypoxia at ERG11 and ERG3. ** P < 0.005. (G and H) ChIP analysis of Set4 under hypoxia in WT and hap1Δ strains at ERG11 and ERG3. ChIP analyses were normalized to DNA input samples and set relative to the untagged WT and the ARS504 loci. Error bars represent SD for three biological replicates with three technical replicates each. Gene expression and ChIP analyses were performed using the FY2609 strain. **** P < 0.0001. N.S., no significant difference.