Figure 4.

daf-19(tm5562) confers decreased roaming behavior and aldicarb resistance. (A) Each 1-day-old adult worm roamed freely from the center of an OP50 bacterial lawn for 1 hr at 19°: shown are representative worm tracks for worms of indicated genotypes. (B) Jitter plot of the proportion of grids crossed versus the total number of grids underneath the OP50 bacterial lawn; N = 30 worms per assay. tm5562 + a indicates transgene expression of daf-19a from its endogenous promoter. The median and 1 SEM are indicated. Tukey’s pairwise comparisons indicate that tm5562 worms roam less than wild-type N2 worms on all assay dates (P < 0.003), while tm5562 + a transgenic worms roam significantly more than tm5562 worms (P < 0.03). (C) Response to 500 μM aldicarb was assessed as in Mahoney et al. (2006). Error bars are 1 SEM. Pairwise comparisons of average paralysis curves (N = 6 assays) were compared (Pyke and Thompson 1986). tm5562 worms are not different from m86 worms (P = 0.56), whereas tm5562 + a transgenic worms differ from m86 worms (P = 0.02) and do not differ from wild-type N2 worms (P = 0.56).