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. 2018 Jan 3;208(3):1083–1097. doi: 10.1534/genetics.117.300571

Figure 5.

Figure 5

Isoform-specific control of daf-19 target genes. (A) Target genes activated by the presence of DAF-19 are still activated in a daf-19(tm5562) background, where DAF-19C, but not DAF-19A, is functional. These genes are also activated when a transgene expressing DAF-19C, but not a DAF-19A transgene, is added to daf-19(m86) null mutant worms. Overexpression of DAF-19A represses expression of asic-2, spg-20, and mapk-15 even in cells where expression is typically independent of daf-19 (Figure 2). N/A = expression of target gene in muscle prevented identification of daf-19a co-injection marker. (B) Two target genes, skr-12 and gakh-1, are repressed in a daf-19 wild-type background, but are not repressed in a daf-19(tm5562) background when DAF-19A is nonfunctional. Expression of rgs-8.1 and mapk-15 is repressed in the presence of either isoform of DAF-19. Bar, 10 μm.